Login
Search
Search
0 Dates
2025
2024
2023
2022
2021
2020
2019
2018
0 Events
CPC 2018
CPC 2019
Curso de Atualização em Medicina Cardiovascular 2019
Reunião Anual Conjunta dos Grupos de Estudo de Cirurgia Cardíaca, Doenças Valvulares e Ecocardiografia da SPC
CPC 2020
CPC 2021
CPC 2022
CPC 2023
CPC 2024
CPC 2025
0 Topics
A. Basics
B. Imaging
C. Arrhythmias and Device Therapy
D. Heart Failure
E. Coronary Artery Disease, Acute Coronary Syndromes, Acute Cardiac Care
F. Valvular, Myocardial, Pericardial, Pulmonary, Congenital Heart Disease
G. Aortic Disease, Peripheral Vascular Disease, Stroke
H. Interventional Cardiology and Cardiovascular Surgery
I. Hypertension
J. Preventive Cardiology
K. Cardiovascular Disease In Special Populations
L. Cardiovascular Pharmacology
M. Cardiovascular Nursing
N. E-Cardiology / Digital Health, Public Health, Health Economics, Research Methodology
O. Basic Science
P. Other
0 Themes
01. History of Cardiology
02. Clinical Skills
03. Imaging
04. Arrhythmias, General
05. Atrial Fibrillation
06. Supraventricular Tachycardia (non-AF)
07. Syncope and Bradycardia
08. Ventricular Arrhythmias and Sudden Cardiac Death (SCD)
09. Device Therapy
10. Chronic Heart Failure
11. Acute Heart Failure
12. Coronary Artery Disease (Chronic)
13. Acute Coronary Syndromes
14. Acute Cardiac Care
15. Valvular Heart Disease
16. Infective Endocarditis
17. Myocardial Disease
18. Pericardial Disease
19. Tumors of the Heart
20. Congenital Heart Disease and Pediatric Cardiology
21. Pulmonary Circulation, Pulmonary Embolism, Right Heart Failure
22. Aortic Disease
23. Peripheral Vascular and Cerebrovascular Disease
24. Stroke
25. Interventional Cardiology
26. Cardiovascular Surgery
27. Hypertension
28. Risk Factors and Prevention
29. Rehabilitation and Sports Cardiology
30. Cardiovascular Disease in Special Populations
31. Pharmacology and Pharmacotherapy
32. Cardiovascular Nursing
33. e-Cardiology / Digital Health
34. Public Health and Health Economics
35. Research Methodology
36. Basic Science
37. Miscellanea
0 Resources
Abstract
Slides
Vídeo
Report
CLEAR FILTERS
Investigating the effects of ß-Estradiol on human microvascular endothelial cells: A focus on sex differences in cardiac health
Session:
SESSÃO DE COMUNICAÇÕES ORAIS 01– DOS LÍPIDOS AOS GENES - FATORES DE RISCO E BIOLOGIA MOLECULAR NA ATEROSCLEROSE E SAÚDE CARDIOVASCULAR
Speaker:
Ivo Fonseca
Congress:
CPC 2025
Topic:
O. Basic Science
Theme:
36. Basic Science
Subtheme:
36.2 Basic Science - Cardiac Biology and Physiology
Session Type:
Comunicações Orais
FP Number:
---
Authors:
Ivo Fonseca; Cristiana Fernandes; Ana Grego; Rita Ferreira; Adelino Leite-Moreira; Marina Dias-Neto; Sandra Marisa Oliveira; Rita Nogueira-Ferreira
Abstract
<p>Endothelial dysfunction plays a key role in the pathogenesis of various conditions, including heart failure with preserved ejection fraction (HFpEF). It has been proposed that coronary microvascular endothelial dysfunction is a central factor driving the structural and functional changes in the heart associated with this syndrome. HFpEF primarily affects post-menopausal women, yet the potential influence of estrogen on its pathophysiology remains poorly understood. We began investigating the effects of β-estradiol (E2) on human microvascular endothelial cardiac cells (HMVECs) from both sexes, focusing on cell proliferation (assessed via the BrdU assay) and the nitric oxide (NO) signaling pathway. HMVECs obtained from commercial sources were exposed to various E2 concentrations (0, 0.01, 0.1, 1, and 10 nM). The results indicated that E2 enhanced proliferation in male HMVECs (p<0.05 for 1 nM E2 compared to 0 nM), while it inhibited proliferation in female cells (p<0.05 for 0.01 nM E2 compared to 0 nM). Nitrite levels in the culture medium appeared to rise in male HMVECs, suggesting that E2 stimulated NO production in these cells. Additionally, E2 treatment seemed to increase the p-eNOS/eNOS ratio in female HMVECs compared to their male counterparts. The levels of estrogen receptor-α did not significantly differ between sexes with E2 treatment, suggesting that this receptor may not play a role in the observed effects. E2 treatment did not affect NOX4 levels, but GPx1 levels appeared consistently higher in male HMVECs across all E2 concentrations. These findings suggest that the impact of E2 on HMVECs can vary in a sex-dependent manner. Future research into the roles of other estrogen receptors and the effects of serum from HFpEF patients on HMVECs may contribute to our understanding of cardiac microvascular endothelial dysfunction in HFpEF.</p>
Slides
Our mission: To reduce the burden of cardiovascular disease
Visit our site